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Image Search Results
Journal:
Article Title: COVALENT REGULATION OF ULVWF STRING FORMATION AND ELONGATION ON ENDOTHELIAL CELLS UNDER FLOW CONDITIONS
doi: 10.1111/j.1538-7836.2008.02991.x
Figure Lengend Snippet: A. ULVWF and plasma VWF multimers were labeled with MPB and immunoprobed with HRP-streptavidin and a polycloncal VWF antibody. Both forms of VWF were labeled with MPB. B. ULVWF and plasma VWF multimers were incubated with the thiol active sepharose 6B beads and the bead captured VWF (containing free thiols) was released by DTT. VWF in supernatant and eluted from the beads was detected by immunoblotting with the polyclonal VWF antibody. The figure is a representative of 6 and 3 separate experiments for plasma VWF and ULVWF, respectively.
Article Snippet: Cell supernatant was collected and the chimera purified through a
Techniques: Labeling, Incubation, Western Blot